CHE 443 Downstream Processing of Bio-products

 

Credit Structure:(3-0)3

 

Catalog Description:

Fundamentals and importance of downstream processing. Recovery, separation and purification of both low and high molecular weight biotechnological products by various methods.

 

Course Objectives:

To give the students the basic principles of separation processes in biotechnological processes; abilities to communicate effectively and function effectively within teams; a solid understanding of professional and ethical responsibility; an awareness of the need for, and ability to engage life-long learning; an understanding of the impact of chemical engineering in a global and environmental context, consistent with the principles of sustainable development; a familiarity with the contemporary chemical engineering issues.

 

Prerequisites:
-

 

Textbook(s):
 

Garcia A.A., Bonen M.R., Ramirez Vick J., Sadaha M., Vuppa A., “Biosepartion Process Science”, Blackwell.

 

Reference:
C.A.Costa and J.S.Cabral, Ed., "Chromatographic and Membrane Processes in Biotechnology"

P.A. Bolter E.L. Cussler and W.S. Hu, "Bioseparations"

M.R. Ladisch, R.C.Willson, C.C. Painton, S.E. Builder., Ed., "Protein Purification"
J.P. Hamel, J.B. Hunter, S.K. Sikdar, Ed., "Downstream Processing" 

 

Syllabus:
 

1.  Introduction                                                                                              (2.5 weeks)    

       1.1  Review of Biological Materials

       1.2  Types of bioproducts involving downstream processes

       1.3.  Characteristics of bioproducts

1.4.    Downstream processing differences between chemical

        and biochemical engineering

2.          Examples of downstream processing sequences for both low

        and high molecular weight products                                                       (0.5 weeks)                                                                          

3.          Common unit operations currently in use for the downstream

        processing of products developed by biochemical processing                (7 weeks)

         3.1  Cell disruption

         3.2  Precipitation

         3.3  Adsorption and ion exchange

         3.4  Chromatography

3.5    Membrane processes

3.6    Electrophoretic methods

4. Term project presentations and discussions                                                  (3 weeks)

 

Homeworks, Quizzes, Projects:

Bi-weekly homeworks are given

 

Computer Usage:

Preparation and presentation of term-project

 

 

Laboratory work:

None

 

Category Content:

Mathematics and Basic Sciences: 0.5 credit

Engineering Sciences: None

Humanities & Social Sciences: None

Departmental: 2.5 credits

 

Instructors:
Ufuk Bakır, Pınar Çalık, Levent Yılmaz